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Polymorphic microsatellite loci in the widespread amphidromous goby Sicyopterus lagocephalus and cross-genus amplification among Sicydiinae |
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| Authors: | Hoareau T B Barbisan F Dubois S Zane L Berrebi P |
| Affiliation: | 1. Laboratoire d’Ecologie Marine, Université de La Réunion, BP7151 2. 9771, Saint‐Denis mes. cedex 9, La Réunion, France;3. T. B. Hoareau and F. Barbisan participated equally to this publication and should be both considered as first authors.;4. Dipartimento di Biologia, Università di Padova, Via Ugo Bassi 58/B, I‐35121 Padova, Italy;5. Institut des Sciences de l’Evolution, UMR 5554 CNRS‐UM2, cc065, Université de Montpellier 2, place Bataillon, 34095 Montpellier, cedex 05, France |
| Abstract: | Microsatellite DNA markers were isolated in an amphidromous goby (Sicyopterus lagocephalus) from a partial genomic library enriched for AC repeats. Eight microsatellites were highly polymorphic with six to 33 alleles per locus and expected heterozygosities ranging from 0.53 to 0.97. Cross-species amplifications were performed within the sub-family Sicydiinae by genotyping individuals from two species of the genus Cotylopus. Some of these loci were successfully amplified and showed polymorphism in the second genus. |
| Keywords: | amphidromous gobiids cross‐genus amplification Genetic markers microsatellite Sicydiins Wahlund effect |
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