Isolation and characterization of microsatellite loci in Babylonia areolata and cross-species amplification in Babylonia formosae habei |
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Authors: | Chen Fei Ke Cai-Huan Wang DE-Xiang Chen Jun Zhan Xin Luo Xuan |
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Institution: | State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China. |
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Abstract: | Nine novel microsatellite primer pairs were presented for Babylonia areolata, representing the first microsatellite markers available for this genus. Levels of polymorphism were variable with 2 to 11 alleles per locus and expected heterozygosities ranging from 0.073 to 0.907 in 27 individuals of the population from which the loci were isolated. We found significant heterozygote deficit at one locus that might be attributable to null alleles. We were successful at cross-amplifying six loci in the congeneric B. formosae habei. These markers are therefore potentially useful for conservation studies, population structure assessment, ecological analyses and linkage map construction. |
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Keywords: | Babylonia Buccinidae microsatellite population genetics |
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