Isolation and characterization of microsatellite loci in the entomopathogenic nematode Heterorhabditis bacteriophora |
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Authors: | Bai Xiaodong Saeb Amr T M Michel Andrew Grewal Parwinder S |
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Affiliation: | Department of Entomology, The Ohio State University - OARDC, 1680 Madison Avenue, Wooster, OH 44691, USA. |
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Abstract: | Twenty microsatellite loci were identified from genomic DNA enrichment and expressed sequence tags of entomopathogenic nematode Heterorhabditis bacteriophora. Eight loci were found to be polymorphic in a Northeast Ohio H. bacteriophora population. Levels of polymorphism were evaluated in 31-56 individuals and the number of alleles ranged from two to three. The values of observed and expected heterozygosities ranged from 0 to 0.536 and from 0.223 to 0.616, respectively. All eight loci showed heterozygote deficiencies, but three conformed to Hardy-Weinberg equilibrium at the subpopulation level. This is the first set of microsatellite markers in entomopathogenic nematodes. |
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Keywords: | entomopathogenic nematodes expressed sequence tags genomic DNA Heterorhabditis bacteriophora microsatellite loci |
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