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Isolation of 11 polymorphic tri- and tetranucleotide microsatellite loci in a North American sedge (Carex scoparia: Cyperaceae) and cross-species amplification in three additional Carex species |
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| Authors: | Hipp Andrew L Kettenring Karin M Feldheim Kevin A Weber Jaime A |
| Institution: | 1. The Morton Arboretum, 4100 Illinois Route 53, Lisle, IL 60532, USA;2. Smithsonian Environmental Research Center, 647 Contees Wharf Road, Edgewater, MD 21037, USA;3. Current address: Department of Watershed Sciences, Utah State University, Logan, UT 84322, USA;4. Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 5. S. Lake Shore Drive, Chicago, IL 60605, USA |
| Abstract: | We report on the isolation and evaluation of 11 microsatellites from a widespread eastern North American wetland sedge, Carex scoparia. Loci exhibit 3-9 alleles over five populations and significant F(IS) (0.204-0.717) in most populations. All primers cross-amplify in at least two other species, and 10 cross-amplify in the more distantly related C. stipata. These markers will be used to examine population genetics and patterns of chromosomal diversification in this ecologically important sedge species and its relatives. |
| Keywords: | Carex subgenus Vignea chromosome diversity Cyperaceae microsatellite enrichment sedges |
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