A cDNA Clone of BcHSP81-4 from the Sterility Line (Pol CMS) of Non-heading Chinese Cabbage (Brassica campestris ssp. chinensis) |
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Authors: | Liu Tongkun Hou Xilin Zhang Jingyi Song Yuping Zhang Shuning Li Ying |
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Institution: | (1) State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China; |
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Abstract: | BcHSP81-4 gene, a member of heat shock proteins, was identified from a suppression subtractive hybridization cDNA library in non-heading
Chinese cabbage (Brassica campestris ssp. chinensis Makino). The deduced amino acid sequence of the BcHSP81-4 cDNA revealed that it has high homology to other plant organelle isoforms and similar homology to both cytoplasmic and prokaryotic
HSP90s. To study the regulation of gene expression, BcHSP81-4 genes in maintainer and sterility lines were monitored at different development stages and at different stress treatments.
Real-time PCR was used for quantification of BcHSP81-4 mRNA. These results indicate that BcHSP81-4 is not responsive to heat shock at least at 35°C, while it is very responsive to salt and cold stress. And high expression
of BcHSP81-4 in the bud of sterile line suggests that it may play prominent roles in sterility of pol CMS in non-heading Chinese cabbage. |
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