Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans |
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| Authors: | Hasan Saba Singh Rana Inder Singh Sandhu Sardul |
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| Institution: | (1) Fungal Biotechnology and Invertebrate Pathology Laboratory, Department of Biological Sciences, R.D. University, Jabalpur, India |
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| Abstract: | A heterologous transformation system was developed for V. lecanii based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate
in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. Mutant no. 01 and 04 was chosen
for the transformation experiments. Plasmid pBT was used as transformation vector containing the Aspergillus nidulans nitrate reductase gene. A frequency of approximately 3 transformants/μg DNA was obtained using the circular vector pBT. The
establishment of a transformation system for V. lecanii is fundamental for genetic manipulation of this microorganism. |
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| Keywords: | Verticillium lecanii Nitrate reductase Heterologous transformation Plasmid pBT |
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