Some Aspects of PCB Metabolism by Horseradish Cells |
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| Authors: | Dr Martina Mackova Ludmila Chroma Petra Kucerova Jiri Burkhard Katerina Demnerova Tomas Macek |
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| Affiliation: | 1. Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology , ICT Prague, Technicka 3, 166 28, Prague 6, Czech Republic;2. Dept. of Environmental Chemistry , ICT Prague, 166 28, Prague 6, Czech Republic;3. Inst. of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic , Flemingovo n. 2, 166 10, Prague 6, Czech Republic |
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| Abstract: | Transformation of polychlorinated biphenyls was studied using different strains of in vitro cultured cells of horseradish (Armoracia rusticana L.). Time and concentration dependence of this process and production of intracellular and extracellular peroxidases were measured with differentiated shooty teratoma culture K54. The yield of PCB transformation and the viability of the cells were highly dependent on PCB concentration. 100 ppm PCB totally inhibited growth of the cells, and reduced their metabolism of xenobiotics. Experiments with a peroxidase (POX) inhibitor, propylgallate, and a cytochrome P450 inhibitor, aminobenztriazole, indicated the involvement of both enzymatic systems in PCB metabolism. |
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| Keywords: | polychlorinated biphenyls PCB horseradish plant cells in vitro shooty teratoma detoxification phytoremediation peroxidase cytochrome P450 inhibitor |
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