Identification of metabolites of phenylacetic acid in rat brain by plasma desorption mass spectrometry |
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Authors: | Yen Hoong Loo Katherine A. Miller Jean Nowlin Marjorie G. Horning |
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Affiliation: | 1. New York State Institute for Basic Research in Mental Retardation, 1050 Forest Hill Road, Staten Island, New York 10314, USA;2. Institute for Lipid Research, Baylor College of Medicine, Houston, Texas 77030, USA |
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Abstract: | Mass spectra of acyl hydroxamates may be obtained directly, without prior derivatization or gas chromatography, by the new technique of plasma desorption mass spectrometry. Authentic alkyl and aryl hydroxamates showed the expected protonated molecular ions when isobutane was used as the carrier gas. Advantage was taken of this novel technique to identify phenylacetyl-CoA and phenylbutyric acid in brain extracts obtained from young rats after a loading dose of phenylacetic acid. The formation of these metabolic products lends strong support to our suggestion that brain dysfunction induced by phenylacetic acid in experimental phenylketonuria may be due to decreased availability of CoA and acetyl-CoA in the rapidly developing brain. |
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