High frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br. |
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Authors: | Mei-Zhu Yang Shi-Rong Jia Eng-Chong Pua |
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Institution: | (1) Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge, 0511 Singapore |
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Abstract: | An efficient tissue culture system for high frequency of plant regeneration from hypocotyl explants of Brassica carinata was developed via manipulation of culture medium and selection of explants. Explants grown on medium containing combinations of 2 mg l-1 BA and 0.01 mg l-1 NAA or 4 mg l-1 kinetin and 0.01 mg l-1 2,4-D regenerated shoots at 100% frequency. High frequency shoot regeneration occurred only from explants originating from 6 to 7-day-old but not younger or older seedlings. Explants showed higher regeneration capacity at the distal end than the proximal end, and the upper segment was more regenerative than the lower segment of hypocotyl. Regenerants were rooted on half-strength growth regulator-free medium, acclimatized and developed into normal, fertile plants.Abbreviations BA
benzyladenine
- 2-4-D
2,4-dichlorophenoxyacetic acid
- NAA
naphthaleneacetic acid
- MS
Murashige & Skoog |
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Keywords: | Brassica carinata Ethiopia mustard hypocotyl plant regeneration tissue culture |
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