SDSL-ESR-based protein structure characterization |
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Authors: | Janez ?trancar Aleh Kavalenka Iztok Urban?i? Ajasja Ljubeti? Marcus A Hemminga |
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Institution: | (1) Laboratory of Biophysics, Solid State Physics Department, “Jožef Stefan” Institute, Jamova 39, 1000 Ljubljana, Slovenia;(2) Laboratory of Biophysics, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands |
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Abstract: | As proteins are key molecules in living cells, knowledge about their structure can provide important insights and applications
in science, biotechnology, and medicine. However, many protein structures are still a big challenge for existing high-resolution
structure-determination methods, as can be seen in the number of protein structures published in the Protein Data Bank. This
is especially the case for less-ordered, more hydrophobic and more flexible protein systems. The lack of efficient methods
for structure determination calls for urgent development of a new class of biophysical techniques. This work attempts to address
this problem with a novel combination of site-directed spin labelling electron spin resonance spectroscopy (SDSL-ESR) and
protein structure modelling, which is coupled by restriction of the conformational spaces of the amino acid side chains. Comparison
of the application to four different protein systems enables us to generalize the new method and to establish a general procedure
for determination of protein structure. |
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Keywords: | |
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