Bio-inorganic Synthesis of ZnO Powders Using Recombinant His-tagged ZnO Binding Peptide as a Promoter |
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Authors: | Lei Song Yingying Liu Zhifang Zhang Xi Wang Jinchun Chen |
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Institution: | (1) College of Life Science and Technology, Beijing University of Chemical Technology, 100029 Beijing, China;(2) The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, China;(3) Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081 Beijing, China;(4) Institute of Chemistry, Chinese Academy of Sciences, 100081 Beijing, China |
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Abstract: | Inorganic-binding peptides termed as genetically engineered polypeptides for inorganics (GEPIs), are small peptide sequences
selected via combinatorial biology-based protocols of phage or cell surface display technologies. Recent advances in nanotechnology
and molecular biology allow the engineering of these peptides with specific affinity to inorganics, often used as molecular
linkers or assemblers, to facilitate materials synthesis, which provides a new insight into the material science and engineering
field. As a case study on this biomimetic application, here we report a novel biosynthetic ZnO binding protein and its application
in promoting bio-inorganic materials synthesis. In brief, the gene encoding a ZnO binding peptide(ZBP) was genetically fused
with His6-tag and GST-tag using E.coli expression vector pET-28a (+) and pGEX-4T-3. The recombinant protein GST-His-ZBP was expressed, purified with Ni–NTA system,
identified by SDS–PAGE electrophoresis and Western blot analysis and confirmed by liquid chromatography-mass spectrometry/mass
spectrometry (LC–MS/MS) analysis. Affinity adsorption test demonstrated that the fusion protein had a specific avidity for
ZnO nanoparticles (NPs). Results from the bio-inorganic synthesis experiment indicated that the new protein played a promoting
part in grain refinement and accelerated precipitation during the formation of the ultra-fine precursor powders in the Zn(OH)2 sol. X-ray diffraction (XRD) analysis on the final products after calcining the precursor powders showed that hexagonal wurtzite
ZnO crystals were obtained. Our work suggested a novel approach to the application about the organic–inorganic interactions. |
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