Application of microsatellite markers developed for arvicoline species in a population genetic study of the root vole <Emphasis Type="Italic">Microtus oeconomus</Emphasis> |
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| Authors: | Magdalena Czajkowska Anetta Borkowska Monika Wieczorek Karol Zub |
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| Institution: | (1) Department of Molecular and Cellular Biology, Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia;(2) Zoological Institute, RAS, Saint-Petersburg, Russia;(3) Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia;(4) Specialized Educational Scientific Center, Novosibirsk State University, Novosibirsk, Russia;(5) Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 OES, UK;(6) Faculty of Science, Department of Biology, University of Ankara, Ankara, Turkey;(7) Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK; |
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| Abstract: | Using a root vole Microtus oeconomus (Pallas, 1776) population in NE Poland we applied 31 microsatellite markers previously developed for root voles and closely
related species, with the aim to improve the population genetic tools in this species. Here we present 16 polymorphic microsatellite
markers grouped into four sets suitable for simultaneous amplification and genetically sex identification in M. oeconomus. The number of alleles per locus in 227 individuals varied from 7 to 26 with a low frequency of null alleles, expected heterozygosity
ranged from 0.758 to 0.927, and observed heterozygosity from 0.722 to 0.947. Two loci showed significant deviation from Hardy-Weinberg
equilibrium (p<0.05) and all loci showed independent inheritance. We expect these markers to be useful for studies of genetic population
structure and kinship of M. oeconomus populations. |
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