Direct plant regeneration from encapsulated nodal segments of Vitex negundo |
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Authors: | N Ahmad and M Anis |
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Institution: | (1) Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, UP, India; |
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Abstract: | An efficient protocol for encapsulation of nodal segments of Vitex negundo L. has been developed for the production of non-embryogenic synthetic seeds. The encapsulations of nodal segments were significantly
affected by the concentrations of sodium alginate and calcium chloride. A 3 % Na2-alginate with 100 mM CaCl2 has been found to be optimum concentration for the production of uniform synthetic seed. For germination, the synseeds were
cultured on Murashige and Skoog (MS) basal medium supplemented with kinetin (KIN) and α-naphthalene acetic acid (NAA) either
singly or in various combinations. MS medium containing 2.5 μM KIN in combination with 1.0 μM NAA was found to be the optimum
for maximum (92.6 ± 3.71 %) plantlet conversion frequency. Well developed regenerated plantlets were hardened, acclimatized
and established in field, where they grew well without any detectable variation. |
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