An improved method for typing of plasma α1-protease inhibitor (PI1) variants in sheep |
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| Authors: | A Estomba M Basaras A Aguirre A Vicario B Jugo L I Mazon |
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| Institution: | Laboratory of Genetics, Department of Animal Biology and Genetics, Faculty of Sciences, University of the Basque Country, Bilbao, Spain |
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| Abstract: | Summary
Sheep plasma α1-protease inhibitor (PI1) variants were typed by analysing neuraminidase treated samples by isoelectric focusing in ultra-thin polyacrylamide gels followed by staining for trypsin inhibition. The 10 different PI1 pheno-types observed were shown to be controlled by four codominant alleles. The probability of sire exclusion provided by PI1 polymorphism was about 0.45 in both of the studied milk sheep breeds (Latxa and Karrantzar) of the Basque Country. |
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| Keywords: | sheep plasma α1-protease inhibitor isoelectric focusing gene frequencies |
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