Expression of the IL-2 gene in the potato (Solanum tuberosum cv. Superior) |
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| Authors: | Yoonkyung Park Baik Hwang Byoungsik Pyo Hyeonsook Cheong |
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| Institution: | (1) Department of Genetic Engineering, Chosun University, Kwang-Ju, Korea;(2) Department of Biology, Chonnam National University, Kwang-Ju, Korea;(3) Department of Food and Biotechnology, Dongshin University, Na-Ju, Korea |
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| Abstract: | We intended to examine the expression of the T-cell growth factor (human interleukin-2) so that a binary vector, pSSK-1, carrying
the IL-2 gene was constructed and transferred intoA. tumefaciens for the purpose of the transformation of the potato (Solanum tuberosum cv. Superior). All of theAgrobacterium-infected potato explants were regenerated to shoots in modified MS medium and 81% of them rooted on the medium containing
kanamycin (200 mg/L). Southern and Northern analysis were performed to verify the transformation events. EL-ISA test indicated
that IL-2 protein was produced from IL-2-transformed potatoes. These results suggested expression and production of the IL-2
protein from the transgenic potato. |
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| Keywords: | T-cell growth factor IL-2 protein potato (Solanum tuberosum cv Superior) modified MS medium expression of the IL-2 gene |
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