二化螟雌蛾对香根草挥发物的EAG和行为反应

【目的】为研发二化螟Chilo suppressalis(Walker)雌蛾植物源引诱剂提供参考。【方法】采用电生理及风洞技术测试了二化螟雌蛾对香根草挥发性物质的定向行为反应。【结果】二化螟雌蛾对供试的16种香根草挥发性物质均能产生EAG反应,且对0.1、1.0、10.0μg/μL 3种浓度下的油酸、胆固醇的EAG反应随浓度升高而增大,对柠檬醛、樟脑、软脂酸的EAG反应随浓度增加而降低。二化螟雌蛾对1.0μg/μL浓度的香根草提取物的EAG反应值为426.11×10?3 Mv,显著高于其他化合物标样(P<0.05)。在风洞中,胆固醇、柠檬醛、正十五酸、正十四碳酸、单棕榈酸甘油、邻苯二甲酸二异辛脂等香根草挥发物对二化螟雌蛾均有引诱作用,并筛选出了具有较好引诱效果的不同配比的混合物,分别为胆固醇︰柠檬醛︰正十四碳酸=33︰24︰11、邻苯二甲酸二异辛脂︰柠檬醛︰单棕榈酸甘油=130︰48︰1、胆固醇︰柠檬醛︰正十四碳酸:单棕榈酸甘油=66︰48︰22︰1、柠檬醛︰单棕榈酸甘油=48︰1。【结论】具有电生理和行为活性的香根草挥发物可作为二化螟雌蛾植物源引诱剂的候选化合物成分。

EAG and behavioral responses ofChilo suppressalis females to plant volatiles fromVetiveria zizanioides

Objectives] To provide a basis for developing botanical attractants for the female rice stem borer,Chilo suppressalis (Walker) (Lepidoptera: Crambidae).Methods]The behavioral responses ofC. suppressalisfemales to 16 plant volatiles substances from vetiver,Vetiveria zizanioiaes,werestudied using an electroantennograph (EAG) and wind tunnel techniques.Results]All 16 substancesinduced EAG responses in female moths. Responses to oleic acid and cholesterol at concentrations of 0.1、1.0 and 10.0μg/μL increased with increasing concentration, but decreased for citral, bornan-2-one and palmitic acid. The EAG value ofC. suppressalis females to vetiverextracts at a concentration of 1.0μg/μL was 426.11×10-3 Mv, significantly higher than that recorded for other standard compounds (P<0.05).C. suppressalisfemales could be attracted byvolatile compounds includingcholesterol, citral, n-pentadecanoic acid, myristic acid, glycerol 1-palmitate and diisooctyl phthalate in wind tunnel assays, and different ratios of these compounds could be ranked in order of decreasing attractiveness to female moths as follows, cholesterol︰citral︰myristic acid=33︰24︰11, diisooctyl phthalate︰citral︰glycerol 1-palmitate=130︰48︰1, cholesterol︰ citral︰ myristic acid: glycerol 1-palmitate=66︰48︰22︰1, citral︰glycerol 1-palmitate=48︰1.Conclusion] Vetivervolatile compounds to whichC. suppressalisfemales displayed electrophysiological and behavioral responses have potential as botanical attractants for these pests.