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Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli |
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| Authors: | Li Yanjun Yan Fangqing Wu Heyun Li Guoliang Han Yakun Ma Qian Fan Xiaoguang Zhang Chenglin Xu Qingyang Xie Xixian Chen Ning |
| Affiliation: | 1.College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China ;2.National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China ;3.Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China ; |
| Abstract: | Journal of Industrial Microbiology & Biotechnology - Although CRISPR/Cas9-mediated gene editing technology has developed vastly in Escherichia coli, the chromosomal integration of large DNA... |
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