Identification of Brachyspira hyodysenteriae-specific DNA fragments using representational difference analysis |
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| Authors: | Rothkamp Anja Strommenger Birgit Gerlach Gerald-F |
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| Institution: | 1. Dstl, Chemical and Biological Sciences, Porton Down, Salisbury SP4 OJQ, UK;2. School of Life and Environmental Sciences, University of Nottingham, Nottingham NG7 2RD, UK;3. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St., London WC1E 7HT, UK;1. Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK;2. Departamento de Microbiolog??a, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain;1. Institut für Hygiene und Mikrobiologie der Universität Würzburg, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany;2. Abteilung für Medizinische Mikrobiologie der Ruhr-Universität Bochum, D-44780 Bochum, Germany;1. Department of Physics, University of California, Los Angeles, CA 90024, USA;1. Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California, USA;2. Department of Radiology, Stanford University School of Medicine, Stanford, California, USA;3. Private practice, San Jose, California, USA;4. Department of Urology, Stanford University School of Medicine, Stanford, California, USA;5. Department of Psychiatry, Stanford University School of Medicine, Stanford, California, USA |
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| Abstract: | Two novel Brachyspira hyodysenteriae-specific DNA fragments, designated as Bh100 and Bh400, were identified using representational difference analysis. To isolate the fragments the combined DNA of the Brachyspira pilosicoli, Brachyspira intermedia, Brachyspira murdochii and Brachyspira innocens reference strains was subtracted from the genome of B. hyodysenteriae strain B204. Both fragments were present in a single copy and mapped to different positions on the genome of B. hyodysenteriae B78(T). Larger fragments encompassing the continuous open reading frames (ORF) of Bh100 and Bh400 were cloned and analysed. Whereas the ORF of 2130 bp encompassing Bh100 did not show homology to any known bacterial protein, Bh400 was part of a putative operon with significant homology to the phosphotransferase system of Bacillus subtilis. |
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| Keywords: | Representational difference analysis Brachyspira hyodysenteriae |
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