Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions |
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| Authors: | Saulo C Bourguignon Wanderley de Souza T Souto-Padrón |
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| Institution: | (1) Instituto de Biologia, CEG, Universidade Federal Fluminense, UFF-Niterói, Rio de Janeiro, Brazil, BR;(2) Laboratório de Ultraestructura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, CCS-Bloco G-UFRJ-Cidade Universitária, 21949-900, Rio de Janeiro, Brazil, BR;(3) Laboratório de Protozoologia I, Instituto de Biofisica Carlos Chagas Filho, CCS-Bloco G-Universidade Federal do Rio de Janeiro, Cidade Universitária, 21949-900, Rio de Janeiro, Brazil e-mail: tcbaeta@ibccf.biof.ufrj.br, Tel.: 55-21-230-7911, Fax: 55-21-260-2364, BR |
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| Abstract: | The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically
defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar
distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy.
The pattern of labeling with the lectins from Triticum vulgaris (WGA), Arachis hypogaea (PNA), Limax flavus (LFA), Canavalia ensiformis (Con-A), and Ricinus communis (RCA-I) significantly changed during the metacyclogenic process. The results obtained are discussed in relation to the role
played by T. cruzi cell surface carbohydrate residues on the process of parasite–host cell interaction.
Accepted: 26 May 1998 |
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