Indole-acetic acid binding proteins in soybean cotyledon |
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Authors: | Mónica Ihl |
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Institution: | (1) Departamento de Ciencias Quimicas y Fisiológicas, Sede Santiago Sur, Universidad de Chile, Casilla 3931, Santiago, Chile;(2) Present address: Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Casilla 15138, Santiago 11, Chile |
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Abstract: | Summary Using the charcoal assay to separate free from bound hormone, the results showed that there is a high binding of IAA to cytosol proteins. Competition experiments were carried out using compounds with different auxin activity (indole-acetic acid, alphanaphtalene acetic acid, indole-butyric acid and phenyl-acetic acid), revealing that specificity of binding exists for those compounds with a molecular configuration appropiate for auxin activity. The protein nature of the indoleacetic acid-binding molecule was demonstrated by the use of enzymes and by its thermolability.Abbreviations IAA
indole-acetic acid
- NAA
alpha-naphtaleneacetic acid
- IBA
indole-butyric acid
- PAA
phenyl-acetic acid |
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