GTP-dependent Ca2+ release from rat liver microsomes. Vesicle fusion is not required |
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| Authors: | J Kleineke A Schr?der H D S?ling |
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| Affiliation: | Abteilung für Klin, Biochemie, Zentrum Innere Medizin der Universit?t, G?ttingen, FRG. |
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| Abstract: | The GTP-dependent calcium release from rat liver microsomes is known to be promoted in the presence of colloids like polyethyleneglycol (PEG), polyvinylpyrrolidine, or albumin. Dawson et al. [(1987) Biochem. J. 244, 87-92] using the 'fusogen' PEG have concluded that both GTP-induced calcium efflux and the enhancement of InsP3-promoted calcium release in the presence of GTP could be attributed to a GTP-dependent vesicle fusion. Here, using the more physiological colloid albumin we report that GTP-induced calcium release from rat liver microsomes may not be linked to vesicle fusion. |
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