首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Alteration of intestinal microbial ecology in mice by recombinant interleuken-2
Authors:Robert L Penn MD  Vinh Q Nguyen MD  Rodney D Berg  Robert D Specian  Paul Stevens PhD
Institution:(1) Medical Service (111 ID), Overton Brooks VA Medical Center, 510 East Stoner Avenue, 71101-4295 Shreveport, LA, USA;(2) VA Medical Center in Shreveport, Louisiana;(3) Department of Microbiology and Immunology, Louisiana State University School of Medicine in Shreveport, Louisiana;(4) Department of Anatomy, Louisiana State University School of Medicine in Shreveport, Louisiana;(5) Cetus Corporation, Emeryville, California, USA;(6) Present address: 1661 Burdette Drive, Suite C, 95121 San Jose, CA, USA;(7) Present address: Pharmacology, Amgen Research Scientist, 1900 Oak Terrace Lane, 91320 Thousand Oaks, CA, USA
Abstract:In addition to its beneficial immunostimulatory properties, interleukin-2 (IL-2) has many significant side effects including gastrointestinal (GI) toxicities. Preliminary studies of the effects of IL-2 on GI bacterial translocation suggested that IL-2 altered intestinal bacterial population levels. In order to further define the effects of IL-2 on intestinal microecology, groups of specific pathogen-free C57BL/6 mice were injected subcutaneously twice daily for 5 days with various dosages of human recombinant IL-2 (up to a maximum of 1.6 mg/kg injection) or an equal volume of sterile buffer. One day after the final injections, IL-2 had significantly (P<.05) increased in a dose-dependent fashion the mean cecal population levels of indigenousEscherichia coli, other gram-negative bacilli, streptococci, and staphylococci. Maximum increases above control cecal population levels were more than 10,000-fold forE. coli and streptococci, 209-fold for gram-negative bacilli other thanE. coli, and 93-fold for staphylococci. These changes were completely reversible, with normal cecal population levels 12 days after the last IL-2 injection. IL-2 did not change the total cecal population levels of strictly anaerobic bacteria. Ileal and cecal structures, as assessed by light microscopy, were not altered by the highest dose of IL-2. When incubated in vivo with 1.6 mg/ml of IL-2, the growth of an indigenous strain ofE. coli was not different from growth in broth alone. Thus, IL-2 treatment caused the intestinal overgrowth of common opportunistic aerobic and facultatively anaerobic bacterial pathogens, without altering total population levels of strict anaerobes or affecting intestinal histology.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号