Immunolocalization of tight junction proteins in the adult and developing human brain |
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Authors: | Email author" target="_blank">Daniela?VirgintinoEmail author Mariella?Errede David?Robertson Carmen?Capobianco Francesco?Girolamo Antonella?Vimercati Mirella?Bertossi Luisa?Roncali |
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Institution: | (1) Department of Human Anatomy and Histology, University of Bari School of Medicine, Bari, Italy;(2) Breakthrough Centre, Institute of Cancer Research, London, UK;(3) Department of Emergency and Organ Transplantation, University of Bari School of Medicine, Bari, Italy;(4) Institute of Obstetric and Gynecology II, University of Bari School of Medicine, Bari, Italy;(5) Department of Biomedical Sciences, University of Foggia School of Medicine, Foggia, Italy |
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Abstract: | The formation of endothelial tight junctions (TJs) is crucial in blood-brain barrier (BBB) differentiation, and the expression and targeting of TJ-associated proteins mark the beginning of BBB functions. Using confocal microscopy, this study analyzed endothelial TJs in adult human cerebral cortex and the fetal telencephalon and leptomeninges in order to compare the localization of two TJ-associated transmembrane proteins, occludin and claudin-5. In the arterioles and microvessels of adult brain, occludin and claudin-5 form continuous bands of endothelial immunoreactivity. During fetal development, occludin and claudin-5 immunoreactivity is first detected as a diffuse labeling of endothelial cytoplasm. Later, at 14 weeks, the immunosignal for both proteins shifts from the cytoplasm to the interface of adjacent endothelial cells, forming a linear, widely discontinuous pattern of immunoreactivity that achieves an adult-like appearance within a few weeks. These results demonstrate that occludin and claudin-5 expression is an early event in human brain development, followed shortly by assembly of both proteins at the junctional areas. This incremental process suggests more rapid establishment of the human BBB, consistent with its specific function of creating a suitable environment for neuron differentiation and neurite outgrowth during neocortical histogenesis.Electronic Supplementary Material Supplementary material is available in the online version of this article at http://dx.doi.org/10.1007/s00418-004-0665-1Daniela Virgintino and Mariella Errede contributed equally to this work |
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Keywords: | Occludin Claudin-5 Tight junctions Blood-brain barrier Human brain development |
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