Nitric Oxide Alleviates Oxidative Damage Induced by Enhanced Ultraviolet-B Radiation in Cyanobacterium |
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Authors: | Lingui Xue Shiweng Li Hongmei Sheng Huyuan Feng Shijian Xu Lizhe An |
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Affiliation: | (1) School of Chemistry and Bioengineering, Lanzhou Jiaotong University, Lanzhou, 730070, People’s Republic of China;(2) School of Life Sciences, Lanzhou University, State Key Laboratory of Arid Agroecology, Lanzhou, 730000, People’s Republic of China |
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Abstract: | To study the role of nitric oxide (NO) on enhanced ultraviolet-B (UV-B) radiation (280–320 nm)-induced damage of Cyanobacterium, the growth, pigment content, and antioxidative activity of Spirulina platensis-794 cells were investigated under enhanced UV-B radiation and under different chemical treatments with or without UV-B radiation for 6 h. The changes in chlorophyll-a, malondialdehyde content, and biomass confirmed that 0.5 mM sodium nitroprusside (SNP), a donor of nitric oxide (NO), could markedly alleviate the damage caused by enhanced UV-B. Specifically, the biomass and the chlorophyll-a content in S. platensis-794 cells decreased 40% and 42%, respectively under enhanced UV-B stress alone, but they only decreased 10% and 18% in the cells treated with UV-B irradiation and 0.5 mM SNP. Further experiments suggested that NO treatment significantly increased the activities of superoxide dismutase (SOD) and catalase (CAT), and decreased the accumulation of O 2 − in enhanced UV-B-irradiated cells. SOD and CAT activity increased 0.95- and 6.73-fold, respectively. The accumulation of reduced glutathione (GSH) increased during treatment with 0.5 mM SNP in normal S. platensis cells, but SNP treatment could inhibit the increase of GSH in enhanced UV-B-stressed S. platensis cells. Thus, these results suggest that NO can strongly alleviate oxidative damage caused by UV-B stress by increasing the activities of SOD, peroxidase, CAT, and the accumulation of GSH, and by eliminating O 2 − in S. platensis-794 cells. In addition, the difference of NO origin between plants and cyanobacteria are discussed. |
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