Effect of temperature on growth of guanidine-resistant mutants of poliovirus

Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temperature when guanidine is included in the culture medium. The data suggest clustering of gr mutations at several sites in the guanidine locus.