Procedures for microencapsulation of enzymes, cells and genetically engineered microorganisms |
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Authors: | Prof T M S Chang S Prakash |
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Institution: | (1) Artificial Cells & Organs Research Centre, McGill University, 3655 Drummond Street, Room 1005, H3G 1Y6 Montreal, Quebec, Canada |
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Abstract: | Methods to microencapsulate enzyme, cells, and genetically engineered cells have been described in this article. More specific
examples of enzyme encapsulation include the microencapsulation of xanthine oxidase for Lesch-Nyhan disease; phenylalanine
ammonia lyase for pheny, ketonuria and microencapsulation of multienzyme systems with cofactor recycling for multistep enzyme
conversions. Methods for cell encapsulation include the details for encapsulating hepatocytes for liver failure and for gene
therapy. This also includes the details of a novel two-step method for encapsulation of high concentrations of smaller cells.
Another new approach is the detailed method of the encapsulation of genetically engineered Escherichia coli DH5 cells for
lowering urea, ammonia, and other metabolites in kidney or, liver failure and other diseases. |
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Keywords: | Artificial cells bioencapsulation microencapsulation nanoencapsulation enzyme cells genetically engineered cells |
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