Alginate synthesis by mucoid strains of Pseudomonas aeruginosa PAO |
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| Authors: | N. H. Piggott I. W. Sutherland T. R. Jarman |
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| Affiliation: | (1) Department of Microbiology, University of Edinburgh, College of Agriculture, West Mains Road, EH9 3JG Edinburgh, UK;(2) Group Research and Development, Tate and Lyle LTD., PO Box 68, RG6 2BX Reading, Berkshire, UK;(3) Present address: Microbiological Chemistry Research Laboratory, The University, NE1 7RU Newcastle-upon-Tyne, UK;(4) Present address: P.A.T. Centre, Cambridge, UK |
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| Abstract: | Summary Alginic acid production by Pseudomonas aeruginosa PAO strains was studied in yeast extract/2% (w/v) gluconate medium. In all of the five strains studied, synthesis of the alginic acid was shown to occur in the stationary phase of growth. Each strain produced similar amounts of alginic acid at both 30° C and 37° C. However the amount of alginic acid varied from 7.5–11.5 gl–1 depending upon the strain. The alginic acid was isolated, purified and its chemical composition determined. All strains produced a polysaccharide rich in polymannuronic acid which contained only polymannuronic acid blocks, polyguluronic acid blocks appeared to be absent. The amount of O-acetylation varied considerably from 2.3–14.7%. Analysis of the chain length distribution by poly-acrylamide gel electropheresis indicated that a homogeneous size of polymer was synthesised when compared to a high viscosity algal sample. |
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