Evidence for selection in cultured diploid fibroblast strains |
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Authors: | Carlos Zavala Gabriel Herner Philip J. Fialkow |
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Affiliation: | 1. Departments of Medicine and Genetics, University of Washington, Seattle, WA 98195, USA;2. Medical Genetics Section, Medical Service, Veterans Hospital, Seattle, WA 98108, USA |
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Abstract: | Single skin fibroblast cultures were established from 29 females heterozygous at the X-linked G-6-PD locus for GdB and an electrophoretic variant, GdA or GdA?. The cultures were transferred serially until they displayed single-enzyme phenotypes (only type A or type B enzyme) or until growth ceased. Single-enzyme phenotypes were observed in 19 instances. This occurred as early as the 4th, and as late as the 38th transfer. Ten cultures evolved A phenotypes and 9 developed B phenotypes. Possible explanations for the development of single-enzyme phenotypes include random cell-sampling during sub-culturing, selection or both. Selection occurring at least in part on the basis of X-linked loci other than G-6-PD seems most likely for those cultures which evolved rapidly to single-enzyme phenotypes, but sampling or selection or both could have played important roles in those cultures which maintained double-enzyme phenotypes for several passages and then rapidly developed single-enzyme phenotypes. In any event, the results indicate that it should not be assumed in investigations using cultivated diploid fibroblasts that the latter are generally representative of the tissue-as-a-whole from which the cultures originate. |
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Keywords: | To whom reprint requests should be addressed. |
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