Identification and mapping of the Gli-R3 locus on chromosome 1R of rye (Secale cereale L.) |
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Authors: | J. M. Carrillo J. F. Vázquez J. Orellana |
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Affiliation: | (1) Department of Genetics, E.T.S. Agricultural Engineering, Universidad Politécnica, E-28040 Madrid, Spain |
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Abstract: | Summary The progenies of two different rye test-crosses were analyzed for secalin proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using unreduced and reduced aqueous ethanol extracts. Segregation for two high-molecular-weight secalin bands (Glu-R1 or Sec3), one -secalin band (Gli-R1 or Sec-1), two 40K -secalin bands (Gli-R1 or Sec1) and two -type secalin bands (new locus) were studied. One recombinant between - and -secalins was found in one test-cross. The new locus, designated Gli-R3 or Sec-4, was mapped between Glu-R1 and Gli-R1, more displaced towards Gli-R1. In test-cross 1 recombination between Glu-R1 and Gli-R3 was 33.80±3.22%, and between Gli-R3 and Gli-R1, 12.04±2.21%. In the other test-cross the map distances were relatively similar but smaller, likely due to less recombination within two different species of Secale. Genes coding for 40K -secalins at Gli-R1 were likely proximal to the centromere with respect to genes coding for -secalins at the same complex locus. |
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Keywords: | Secalin loci Linkage mapping Chromosome 1R Secale cereale |
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