Screening of a broad range of rice (Oryza sativa L.) germplasm for in vitro rapid plant regeneration and development of an early prediction system |
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Authors: | A. N. G. Dabul H. Belefant-Miller M. RoyChowdhury J. F. Hubstenberger A. Lorence G. C. Phillips |
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Affiliation: | (1) Arkansas Biosciences Institute and College of Agriculture and Technology, Arkansas State University, P.O. Box 1080, State University, AR 72467, USA;(2) USDA-ARS, Dale Bumpers National Rice Research Center, 2890 Hwy 130 E, Stuttgart, AR 72160, USA;(3) Arkansas Biosciences Institute and Department of Chemistry and Physics, Arkansas State University, P.O. Box 639, State University, AR 72467, USA |
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Abstract: | Rice has emerged as a model monocot for studies in agriculture and biotechnology due to its relatively small genome and a ready accessibility to plant material. Tissue culture is one of the tools required for genetic transformation and some breeding programs, and the selection of high-frequency regenerator types is essential for success in these technologies. Thirty-three rice entries with agricultural and biotechnological characteristics of interest were screened with the aim to identify the best regenerators. Entries that exhibited between 50% and 90% regeneration frequencies include ‘Taipei-309,’ ‘Super Dwarf,’ ‘Norin’ (japonica types), PI 312777, ‘Ali Combo’ (indica types), ‘STG-S,’ and ‘LA3’ (red rice types). One third of the entries tested were at least two times better at regeneration than the often-cited regenerator ‘Nipponbare.’ Those entries showing at least 85% frequency of greening or somatic embryo formation at 15 or 30 d on regeneration medium ultimately produced whole plants after 45 d on regeneration medium at high frequency (at least 40%); those entries not reaching the 85% threshold of greening by Days 15 or 30 exhibited moderate (15–40%) to low (less than 10%) frequency of whole plant regeneration. This greening response suggests the means for an early prediction system for identification of useful rice regenerator lines, which would be beneficial for high-throughput screening of germplasm as well as for decreasing the time and cost of in vitro culture. |
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Keywords: | Tissue culture Rice Early prediction Plant regeneration |
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