| 裂解型腺病毒Ad-E1A对人脐静脉内皮细胞的影响 |
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| 引用本文： | 叶震敏 汪小华 钟江 缪竞诚 盛伟华 谢宇锋 王金志 杨吉成. 裂解型腺病毒Ad-E1A对人脐静脉内皮细胞的影响[J]. 生物工程学报, 2006, 22(3): 397-402 |
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| 作者姓名： | 叶震敏 汪小华 钟江 缪竞诚 盛伟华 谢宇锋 王金志 杨吉成 |
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| 作者单位： | 1. 苏州大学医学院细胞与分子生物学教研室,苏州,215123
2. 复旦大学生命科学院微生物系,上海,210003 |
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| 基金项目： | 苏州大学校科研和教改项目 |
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| 摘 要： | 以QBI-293A细胞基因组DNA为模板,PCR扩增E1A基因,酶切连接到pAdTrack-CMV转移质粒上,pAdTrack-CMV-E1A经PmeI线性化后,与pAdEasy-1共转化大肠杆菌BJ5183,筛选重组腺病毒质粒pAdEasy-1-pAdTrack-CMV-E1A,经PacI线性化,脂质体转染QBI-293A细胞,获得裂解型腺病毒Ad-E1A。裂解型腺病毒Ad-E1A在ECV304细胞内复制裂解,抑制细胞的生长,并可以降低VEGF的表达,探讨了Ad-E1A可能通过抑制ECV304细胞NF-κB的激活而引起细胞生长抑制的机制,说明Ad-E1A具有抑制肿瘤转移的功能。
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| 关 键 词： | 裂解型腺病毒 Ad-E1A 人脐静脉内皮细胞 |
| 文章编号： | 1000-3061(2006)03-0397-06 |
| 收稿时间： | 2005-11-28 |
| 修稿时间： | 2006-02-17 |
The Effect of Oncolytic Adenovirus on Human Umbilical Vein Endothelial Cell |
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| YE Zhen-Min,WANG Xiao-Hua,ZHONG Jiang,MIAO Jing-Cheng,SHENG Wei-Hua,XIE Yu-Feng,WANG Jin-Zhi,YANG Ji-Cheng. The Effect of Oncolytic Adenovirus on Human Umbilical Vein Endothelial Cell[J]. Chinese journal of biotechnology, 2006, 22(3): 397-402 |
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| Authors: | YE Zhen-Min WANG Xiao-Hua ZHONG Jiang MIAO Jing-Cheng SHENG Wei-Hua XIE Yu-Feng WANG Jin-Zhi YANG Ji-Cheng |
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| Affiliation: | 1 Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China 2 Microbiology Department, College of Life Science, Fudan University, Shanghai 210003, China |
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| Abstract: | The E1A gene was obtained by PCR with QBI-293A cell genome DNA as template. After enzyme digestion, the E1A gene was ligated to transfer vector pAdTrack-CMV. The positive clone pAdTrack-CMV-E1A were lineared by PmeI and cotransformed with pAdEasy-1 in BJ5183 E. coli. The recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-E1A were digested by PacI and transfected into QBI-293A cells with liposomes. The oncolytic recombinant adenovirns Ad-E1A was obtained after 7 days. The results showed that this oncolytic adenovirns Ad-E1A can replicate in ECV304 cells and inhibit growth of ECV304 cell. In addition, it also decreased the secretion of VEGF and expression of NF-kB of ECV304 cells, indicating that AdE1A have potential of inhibition of tumor metastasis. |
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| Keywords: | oncolytic adenovirus Ad-E1A human umbilical vein endothelial cell |
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