| 双歧杆菌对人大肠癌HT_(29)细胞谷胱甘肽巯基转移酶基因的诱导表达 |
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| 引用本文： | 唐立,赵宝昌,崔秀云.双歧杆菌对人大肠癌HT_(29)细胞谷胱甘肽巯基转移酶基因的诱导表达[J].中国微生态学杂志,2000(1). |
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| 作者姓名： | 唐立 赵宝昌 崔秀云 |
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| 作者单位： | 大连医科大学微生态研究所!大连116027(唐立，赵宝昌)，大连医科大学生化教研室(崔秀云) |
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| 摘 要： | 应用北杂交技术研究双歧杆菌对大肠癌HT29细胞谷胱甘肽巯基转移酶基因的诱导表达发现:双歧杆菌及其发酵过滤液在作用HT29细胞2小时后,谷胱甘肽巯基转移酶基因的mRNA分别提高1.4倍和1.3倍,提示诱导谷胱甘肽巯基转移酶基因的高效表达,阻断外来致癌物的亲核攻击可能是双歧杆菌抗肿瘤的一个分子机制。
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| 关 键 词： | 双歧杆菌 谷胱甘肽巯基转移酶 基因 |
GENE INDUCTIVE EXPRESSION OF BIFIDOBACTERIUM ON GLUTATHIONE S-TRANSFERASE IN HT_(29) CELL LINE |
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| Tang Li,Zhao Baochang,Cui Xou Yin..GENE INDUCTIVE EXPRESSION OF BIFIDOBACTERIUM ON GLUTATHIONE S-TRANSFERASE IN HT_(29) CELL LINE[J].Chinese Journal of Microecology,2000(1). |
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| Authors: | Tang Li Zhao Baochang Cui Xou Yin |
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| Abstract: | The Northern hybridization technique was applied to study the effect of Bifidobacterium on the inductive gene expression of glutathione S-transferase. It has been found that the level of glutathione S-transferase (GSTs) mRNA increases 1 4 fold, 1 3 fold respectively,when HT 29 cells were exposed 2 hours to Bifidobacterium and filtration of fermentation. It was indicated a molecular mechanism of Bifidobacterium antitumor in that GST gene is inductive expression and preventing Xenbiotics nucleophilic attack. |
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| Keywords: | Bifidobacterium Glutathione S-transferase Gene |
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