Electron microscopic analysis of in vitro transposition intermediates of bacteriophage Mu DNA |
| |
| Authors: | J L Miller G Chaconas |
| |
| Institution: | 1. Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, CA, USA;2. Division of Gastroenterology and Hepatology, Department of Medicine, University of California, San Diego, La Jolla, CA, USA;3. Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA;4. Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA;5. School of Physics and Astronomy, Rochester Institute of Technology, Rochester, NY, USA;6. Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development, University of Florida, Gainesville, FL, USA;7. Center for Microbiome Innovation, University of California, San Diego, La Jolla, CA, USA;8. Synthetic Biology Institute, University of California, San Diego, La Jolla, CA, USA;9. Molecular Biology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA;10. Jennifer Moreno Department of Veterans Affairs, La Jolla, CA, USA;11. Moores Cancer Center, University of California, San Diego, La Jolla, CA, USA;12. Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA;13. Shu Chien-Gene Lay Department of Bioengineering, University of California San Diego, La Jolla, CA, USA;1. Centre for Bacterial Resistance Biology, Imperial College London, London, UK;2. Department of Biology, University of Oxford, Oxford, UK;3. Parasites and Microbes Programme, Wellcome Sanger Institute, Cambridge, UK |
| |
| Abstract: | Bacteriophage Mu is a highly efficient transposon and the only moveable element for which an in vitro transposition system has been reported. Recently, this system has been used by Craigie and Mizuuchi Cell 41 (1985) 867-876] to identify and biochemically characterize intermediates in the transposition process. We have utilized the in vitro transposition system to generate intermediates in the transposition process and have analyzed these intermediates by electron-microscopic methods. Partial denaturation mapping has shown the intermediates to be theta-shaped structures in which the phi X174 target DNA is joined to the mini-Mu plasmid at the ends of the Mu genome. Our results are in agreement with the previous biochemical studies and the type of intermediate we observe is exactly what is predicted by the Shapiro model of transposition Proc. Natl. Acad. Sci. USA 76 (1979) 1933-1937]. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
