Characterisation of the RNA binding properties of the coronavirus infectious bronchitis virus nucleocapsid protein amino-terminal region |
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Authors: | Spencer Kelly-Anne Hiscox Julian A |
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Institution: | Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UK. |
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Abstract: | The coronavirus nucleocapsid (N) protein binds viral RNA to form the ribonucleocapsid and regulate RNA synthesis. The interaction of N protein with viral RNA was investigated using circular dichroism and surface plasmon resonance. N protein underwent a conformational change upon binding viral RNA and the data indicated electrostatic interactions were involved in the binding of the protein to RNA. Kinetic analysis suggested the amino-terminal region facilitates long-range non-specific interactions between N protein and viral RNA, thus bringing the RNA into close proximity to N protein allowing specific contacts to form via a 'lure' and 'lock' mechanism. |
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Keywords: | Coronavirus RNA binding Nucleocapsid protein IBV Surface plasmon resonance Circular dichroism |
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