Nicotianamine Over-accumulation Confers Resistance to Nickel in <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> |
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Authors: | Katia?Pianelli Stéphane?Mari Laurence?Marquès Michel?Lebrun Email author" target="_blank">Pierre?CzernicEmail author |
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Institution: | (1) Biochimie & Physiologie Moléculaire des Plantes, UMR5004, Centre National de la Recherche Scientifique, Institut National pour la Recherche Agronomique, Ecole Nationale Supérieure d’Agronomie et Université Montpellier 2, cedex 5, F-34095, cc002, Montpellier, France |
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Abstract: | Nicotianamine is a methionine derivative involved in iron homeostasis, able to bind various other metals in vitro. To investigate its role in vivo, we expressed a nicotianamine synthase cDNA (TcNAS1) isolated from the polymetallic hyperaccumulator Thlaspi caerulescens in Arabidopsis thaliana. Transgenic plants expressing TcNAS1 over-accumulated NA, up to 100-fold more than wild type plants. Furthermore, increased NA levels in different transgenic
lines were quantitatively correlated with increased nickel tolerance. The tolerance to nickel is expressed at the cellular
level in protoplast experiments and is associated with an increased NA content. We have also shown that the most NA-over accumulating
line showed a high tolerance to nickel and a significant Ni accumulation in the leaves when grown on nickel-contaminated soil.
Our results highlight a new potential role for nicotianamine in heavy metal tolerance at the cellular but also at the whole
plant level, easily transposable to a non-tolerant non-hyperaccumulator species. These results open new perspectives for the
modulation of nicotianamine content in plants for phytoremediation. |
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Keywords: | Nicotianamine nickel resistance phytoremediation Thlaspi caerulescens |
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