基于广泛靶向代谢组学的竹黄活性成分分析

竹黄是我国一种重要的药用真菌,在医学、农业、食品等方面应用广泛且前景可观。为深入挖掘竹黄中有药理活性的有效化学成分,了解其在生长发育过程中不同时期代谢物的变化规律,利用广泛靶向代谢组学技术检测了竹黄子座不同发育时期的代谢物,找出差异代谢物并进行代谢通路分析。从竹黄子座中共检测出612种代谢物,前期和中期特有27种代谢物。黄酮类、奎宁酸、香豆素等具有良好生物活性的化合物首次在竹黄中被检测到。筛选出的差异代谢物主要是脂质、氨基酸、核苷酸、黄酮类、萜类、有机酸等物质,其中黄酮和氨基酸类化合物占主要地位。通过对代谢通路富集分析,获得6条具有显著意义的代谢途径。黄酮类化合物被认为是除竹红菌素外与竹黄药效有重要联系的化合物。本研究为竹黄药用机理及有效成分深入研究提供了一定的理论基础,为竹黄有效成分的代谢途径解析提供参考。

Widely-targeted metabolomics analysis of Shiraia bambusicola bioactive components

Shiraia bambusicola is an important medicinal fungus in China, which has a wide applications in medicine, agriculture, food and other fields. The technique of widely-targeted metabolomics was used to detect metabolites produced by Shiraia bambusicola at various developmental stages for identifying differential metabolites and performing metabolic pathway analysis. A total of 612 metabolites were identified from Shiraia bambusicola, of which 27 unique metabolites were found in the early and medium developmental stages. Flavonoids, quinic acid, coumarin and other compounds with notable biological activity were detected for the first time. The differential metabolites selected mainly include lipid, amino acids, nucleotide, flavonoids, terpenoids, organic acids, etc. Among them, flavonoids and amino acids were prior to the others. Six significant metabolic pathways were obtained through enrichment analysis. Flavonoids are considered to be important compounds that are related to the medicinal value of Shiraia bambusicola in addition to hypocrellin. This result provides a basis for further study of the medicinal mechanism and active ingredients of Shiraia bambusicola.