| 大白菜腐霉根腐病的病原 |
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| 引用本文： | 郭梅,周亚男,肖倩,李宝笃,梁晨.大白菜腐霉根腐病的病原[J].菌物学报,2019,38(6):761-767. |
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| 作者姓名： | 郭梅 周亚男 肖倩 李宝笃 梁晨 |
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| 作者单位： | 青岛农业大学植物医学学院山东省植物病虫害综合防控重点实验室 山东青岛266109;青岛农业大学植物医学学院山东省植物病虫害综合防控重点实验室 山东青岛266109;山东省应用真菌重点实验室 山东青岛266109 |
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| 基金项目： | 国家重点研发计划项目（2017YFD0201104）;青岛市现代农业产业技术体系项目（6622316110） |
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| 摘 要： | 在山东青岛发现一种新的大白菜根腐病,主根下部腐烂,根部维管束变褐变黑,地上部生长缓慢,矮小,甚至死亡。通过组织分离法和单菌丝分离法获得纯化菌株HMQAU170164。经柯赫氏法则验证,综合该病原菌形态学特征、生物学特性、ITS和coxⅠ序列分析,界定其为芸薹腐霉Pythium brassicum。该菌引起的大白菜腐霉根腐病为首次报道。
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| 关 键 词： | 芸薹腐霉 形态学 分子鉴定 黑根病 麦粒-沙培法 |
| 收稿时间： | 2018-12-24 |
The pathogen causing Pythium root rot of Chinese cabbage |
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| Authors: | GUO Mei ZHOU Ya-Nan XIAO Qian LI Bao-Du LIANG Chen |
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| Institution: | 1. Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, China2. Shandong Provincial Key Laboratory of Applied Mycology, Qingdao, Shandong 266109, China |
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| Abstract: | As a new disease, Chinese cabbage root rot was found in Qingdao, Shandong Province. The lower part of taproot becomes rot, and the root vascular bundle turns brown and black. The overground plant grows slowly, dwarfs, and even dies. The pure isolate HMQAU170164 of the pathogen was obtained by tissue isolation method and single mycelium isolation method. Pathogenicity test by Koch?s postulate, observation of morphological and biological characteristics, and sequence analyses of ITS and coxⅠ, proved that the pathogen was Pythium brassicum. It is the first report of P. brassicum as the pathogen of Chinese cabbage root rot. |
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| Keywords: | Pythium brassicum morphology molecular identification black root wheat grain sand culture method |
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