正交-满意度函数优化灵芝多糖及三萜共提取条件及其清除自由基活性

灵芝Ganoderma lingzhi是我国的一种著名珍稀药用真菌,多糖及三萜类化合物是其主要活性成分提。取乙时醇间水4h溶和液料为液提比取1:剂40,,以多灵糖芝和多三糖萜及的三提萜取提率取分率别为达响到应1值.13,=%正?和交k0-.4满6意%。度优函化数条优件化下乙提醇取含的量多、糖提和取三温萜,前者DPPH自由基清除活性高于热水法提取多糖,后者与乙醇浸提法提取三萜有相近的DPPH自由基清除活性。

Optimization of co-extraction of polysaccharides and triterpenoids from Ganoderma lingzhi by orthogonal design coupled with desirability and measurement of free radical scavenging activities of polysaccarides and triterpenoids extracted

Ganoderma lingzhi is a precious and famous traditional Chinese medicinal fungus. Polysaccharides and triterpenoids are the primary bioactive components of this fungus. In the present study, ethanol-water mixture was adopted for extraction of polysaccarides and triterpenoids from G. lingzhi. Based on the extraction rate, four extraction factors including enthanol concentration, extraction temperature, extraction duration and solid to liquid ratio were optimized using orthogonal array design coupled with desirability function. The optimal extraction conditions were obtained viz. ethanol concentration of 40%, extraction temperature of 95°C, extraction duration of 4h, and solid to liquid ratio of 1:40. Under such an optimization, the extraction rate of polysaccharides and triterpeonids reached 1.13% and 0.46%, respectively. DPPH radical scavenging activity of the polysaccharides obtained under the optimized conditions was higher than that obtained by hot water extraction. There was no difference between triterpenoids isolated from the optimized co-extraction and those from ethanol extraction.