慢性间歇性缺氧降低SD大鼠精子数量和活力的研究

摘要 目的：通过制备大鼠慢性间歇性缺氧的动物模型，观察间歇性缺氧对雄性大鼠精子数量和活力的影响，并初步探讨其可能的发生机制。方法：16只雄性SD大鼠随机分为对照组和缺氧组。间歇性缺氧8周后分别测定两组大鼠精子活力和精子浓度，睾丸组织中P53的表达量和细胞的凋亡水平，以及血清睾酮（T）、促卵泡素（FSH）和黄体生成素（LH）的浓度。结果：与对照组比较，缺氧组大鼠精子的浓度和活力均降低（P>0.05)，并且前向运动的精子数明显减少（P<0.05）。同时，缺氧组睾丸组织P53 mRNA的表达和睾丸组织中凋亡的细胞均多于对照组，血清T、LH的浓度低于对照组（P<0.05）。结论：间歇性缺氧时可能影响了下丘脑-垂体-性腺轴，不仅使睾酮合成减少，还降低了对P53的抑制作用而诱导了大量细胞的凋亡，最终降低了SD大鼠的精子数量和活力。

Study on the Decrease of Sperm Count and Motility in SD Rats by Chronic Intermittent Hypoxia

ABSTRACT Objective: By replicating the model of chronic intermittent hypoxia, the effects of intermittent hypoxia on sperm count and motility in SD rats were observed and the possible mechanism was discussed. Methods: Sixteen male SD rats were randomly divided into control group and hypoxia group. After 8 weeks of intermittent hypoxia, sperm motility and sperm concentration, expression of P53 and cell apoptosis level, as well as serum concentrations of testosterone (T), follicle- stimulating hormone (FSH) and luteinizing hormone (LH) were measured. Results: Compared with the control group, the sperm concentration and motility of rats in the hypoxia group were decreased(P>0.05), and the sperm in the forward movement was significantly decreased(P<0.05). At the same time, the expression of P53 mRNA and apoptosis cells in the hypoxia group more than those in the control group, but the concentration of serum T and LH was lower than that in the control group(P<0.05). Conclusion: Intermittent hypoxia may effect the hypothalamic-pituitary-gonadal axis, which not only reduces the synthesis of testosterone, but also reduces the inhibitory effect on P53 and induces the apoptosis of a large number of cells, ultimately decreasing the sperm count and motility in SD rats.