Resistance to diamide insecticides in diamondback moth,Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor |
| |
Authors: | Bartek Troczka Christoph T Zimmer Jan Elias Corinna Schorn Chris Bass TG Emyr Davies Linda M Field Martin S Williamson Russell Slater Ralf Nauen |
| |
Institution: | 1. Department of Entomology, National Chung Hsing University, 145 Xingda Rd., Taichung 40227, Taiwan ROC;2. Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, 11, Guangming Rd., Wufong, Taichung 41358, Taiwan ROC |
| |
Abstract: | Diamide insecticides such as chlorantraniliprole and flubendiamide are a new class of insecticide that selectively target insect ryanodine receptors (RyR), a distinct class of homo-tetrameric calcium release channels which play a pivotal role in calcium homeostasis in numerous cell types. Resistance to these insecticides has recently been reported in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae), a global lepidopteran pest of cruciferous crops. In the present study a region of the gene encoding the proposed diamide binding site of the RyR from P. xylostella collected from the Philippines and Thailand and found to be over 200-fold resistant to both chlorantraniliprole and flubendiamide compared to susceptible strains, were amplified by RT-PCR and sequenced. Comparison of the sequence with those from several susceptible reference strains revealed non-synonymous mutations in each of the resistant strains that in both cases lead to a glycine to glutamic acid substitution (G4946E) in the protein. The independent evolution of the same amino acid substitution within a highly conserved region of the proposed diamide binding site in two geographically separated resistant strains of P. xylostella strongly suggests a causal association with diamide resistance. Furthermore we designed a pyrosequencing-based diagnostic assay for resistance monitoring purposes that can be used to detect the G4946E mutation in field-collected samples of diamondback moth. The implications of the reported findings for resistance management strategies are discussed. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|