AMPKα1 Deletion Shortens Erythrocyte Life Span in Mice: ROLE OF OXIDATIVE STRESS* |
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Authors: | Shaobin Wang George L Dale Ping Song Benoit Viollet Ming-hui Zou |
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Institution: | From the ‡Division of Endocrinology and Diabetes.;¶Division of Hematology, Department of Medicine, and ;§Department of Physiology, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104.;‖Institut Cochin, 75014 Paris, France.;the **University Paris Descartes, CNRS (UMR 8104), 75014 Paris, France, and ;‡‡Inserm, U567 Paris, France |
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Abstract: | AMP-activated protein kinase (AMPK) is an energy sensor essential for maintaining cellular energy homeostasis. Here, we report that AMPKα1 is the predominant isoform of AMPK in murine erythrocytes and mice globally deficient in AMPKα1 (AMPKα1−/−), but not in those lacking AMPKα2, and the mice had markedly enlarged spleens with dramatically increased proportions of Ter119-positive erythroid cells. Blood tests revealed significantly decreased erythrocyte and hemoglobin levels with increased reticulocyte counts and elevated plasma erythropoietin concentrations in AMPKα1−/− mice. The life span of erythrocytes from AMPKα1−/− mice was less than that in wild-type littermates, and the levels of reactive oxygen species and oxidized proteins were significantly increased in AMPKα1−/− erythrocytes. In keeping with the elevated oxidative stress, treatment of AMPKα1−/− mice with the antioxidant, tempol, resulted in decreased reticulocyte counts and improved erythrocyte survival. Furthermore, the expression of Foxo3 and reactive oxygen species scavenging enzymes was significantly decreased in erythroblasts from AMPKα1−/− mice. Collectively, these results establish an essential role for AMPKα1 in regulating oxidative stress and life span in erythrocytes. |
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Keywords: | AMP-activated kinase (AMPK) Antioxidant Erythrocyte Oxygen Radicals Reactive Oxygen Species (ROS) |
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