补肾填精法对脑卒中失眠症模型大鼠血清褪黑素含量表达的影响

目的:探讨补肾填精法对脑卒中失眠症模型大鼠血清褪黑素(Melatonin,MT)含量表达的影响。方法:24只建模成功的脑卒中失眠症大鼠随机平分为3组-模型组、低剂量组、高剂量组。于造模第7 d起,三组分别给予生理盐水及补肾填精汤(低剂量组最终含生药浓度低剂量为0.7 g/mL、中剂量为1.4 g/mL)以1 mL/(100 g·d)灌胃,连续14 d,记录血清MT表达变化情况。结果:三组治疗第1 d、第7 d与第14 d的逃避潜伏期在组内与组间对比差异无统计学意义(P>0.05)。低剂量组、高剂量组治疗第7 d与第14 d的进入中央区次数多于模型组(P<0.05),也多于治疗第1 d(P<0.05),高剂量组也多于低剂量组(P<0.05)。低剂量组、高剂量组治疗第14 d的血清MT含量、去甲肾上腺素转运蛋白与褪黑素受体蛋白相对表达水平高于模型组(P<0.05),高剂量组高于低剂量组(P<0.05)。结论:补肾填精法在脑卒中失眠症模型大鼠中的应用能促进血清MT的释放,提高去甲肾上腺素转运蛋白与褪黑素受体蛋白的表达,从而促进缓解失眠症状。

Effect of Tonifying Kidney and Replenishing Essence Method on Expression of Serum Melatonin in Stroke and Insomnia Model Rats

Objective:To investigate the effect of the method of invigorating the kidney and replenishing essence on the expression of serum melatonin(MT)in rat model of stroke and insomnia.Methods:A total of 24 rats of successfully modeled stroke and insomnia were randomly and equally divided into three groups-model group,low-dose group,and high-dose group.On the 7th day of model establishment,the three groups were given physiological saline and Tonifying Kidney and Replenishing Essence Decoction(the final low-dose group containing crude drug concentration was 0.7 g/mL,the medium dose was 1.4 g/mL)at a rate of 1 mL/(100 g·d)Gavage for 14 consecutive days.The changes in serum MT expression were recorded.Results:There was no significant difference in the escape latency among the three groups on the 1st,7th and 14th day of treatment(P>0.05).The low-dose group and high-dose group had more access to the central area on the 7th and 14th day of treatment than the model group(P<0.05),and more than the 1st day of treatment(P<0.05),and the high-dose group was also more than the low-dose group(P<0.05).The serum MT content,the relative expression levels of norepinephrine transporter and melatonin receptor protein on the 14th day of treatment in the low-dose and high-dose groups were higher than those in the model group(P<0.05),and the high-dose group was higher than the low-dose group(P<0.05).Conclusions:The application of the method of Tonifying Kidney and Replenishing Essence in stroke and insomnia model rats can promote the release of serum MT,increase the expression of norepinephrine transporter and melatonin receptor protein,thereby alleviating insomnia symptoms.