黑色素瘤患者血浆miR-21表达与临床病理特征的相关性

摘要 目的：探讨黑色素瘤患者血浆miR-21表达与临床病理特征的相关性。方法：2018年2月到2019年5月选择在本院诊治的黑色素瘤患者121作为黑色素瘤组，另选取同期良性皮肤肿瘤患者121例作为良性组。采集患者的空腹静脉血并提取血浆，采用qRT-PCR 技术检测血浆miR-21表达。调查患者的临床病理特征并进行相关性分析。结果：黑色素瘤组的血浆miR-21相对表达水平显著高于良性组(P<0.05)。在黑色素瘤组中，血浆miR-21相对表达水平与患者的年龄、性别、饮酒、肿瘤部位等无相关性(P>0.05)，与临床分期、肿瘤转移、溃疡、吸烟等有显著相关性(P<0.05)。取miR-21诊断黑色素瘤的临界值(4.71)，其诊断黑色素瘤的敏感性、特异性、准确性分别为77.6 %、87.0 %和81.7 %。logistic 回归模型分析显示吸烟、溃疡、miR-21表达水平都为影响黑色素瘤发生的重要因素(P<0.05)。结论：miR-21变化在黑色素瘤中呈现高表达，且与临床特征显著相关性，早期鉴别诊断黑色素瘤的预后效果比较好，同时有助于提高患者的生存率与生活质量。

Correlation between Plasma miR-21 Expression and Clinicopathological Characteristics in Patients with Melanoma

ABSTRACT Objective: To explore the correlation between plasma miR-21 expression and clinicopathological characteristics in patients with melanoma. Methods: From February 2018 to May 2019, 121 cases of patients with melanoma diagnosed in our hospital were selected as the melanoma group, and 121 cases of patients with benign skin tumors in the same period were selected as the benign group. The fasting venous blood of the patients were collected and plasma were extracted, and the plasma miR-21 expression were detected by qRT-PCR technology. And were to investigate the clinicopathological characteristics of patients and were given correlation analysis. Results: The relative expression level of plasma miR-21 in melanoma group were significantly higher than that in benign group (P<0.05). In the melanoma group, the relative expression level of plasma miR-21 were not related to the patient''s age, sex, drinking, tumor location, etc. (P>0.05), and were significantly correlated with clinical stage, tumor metastasis, ulcer, and smoking (P<0.05). When selected the critical values (4.71) for miR-21 diagnosis of lung cancer, the sensitivity, specificity, and accuracy of the diagnosis of lung cancer were 77.6 %, 87.0 %, and 81.7 %, respectively. Logistic regression model analysis showed that smoking, ulcers, and miR-21 expression levels were important factors affected melanoma occurrence (P<0.05). Conclusion: The changes of miR-21 are highly expressed in melanoma and have significant correlation with clinical features. The early differential diagnosis of melanoma have better effect, and it helps to improve the survival rate of patients.