多巴胺联合NE对感染性休克致急性肝损伤大鼠肝功能、炎性因子及NF-κB p65蛋白的影响

目的:本研究通过研究多巴胺(Dopamine,DA)和去甲肾上腺素(Noradrenaline,NE)对感染性休克致急性肝损伤大鼠肝功能、炎性因子及NF-NF-κB p65蛋白的影响,以期为临床治疗提供一定的试验依据。方法:以48只SPF级健康雄性SD大鼠为研究对象,根据随机数字表法分为四组,每组12只,分别为对照组,脂多糖(lipopolysaccharide,LPS)组,NE组,DA+NE组。LPS、NE和DA+NE建立感染性休克模型,NE组静脉输注去甲肾上腺素,DA+NE组在NE组的基础上静脉输注DA。对各组大鼠肝功能、炎性因子和NF-κB p65蛋白水平进行检测。结果:与对照组相比,LPS、NE和DA+NE组血清天冬氨酸转氨酶(aspartate transaminase,AST)和丙氨酸转氨酶(alanine Transaminase,ALT)水平均显著升高(P<0.05),与LPS组相比,NE和DA+NE组大鼠血清AST和ALT均有不同程度的降低(P<0.05),与NE组相比,DA+NE组大鼠血清AST和ALT水平降低更显著(P<0.05)。与对照组相比,LPS、NE和DA+NE组血清白细胞介素6(interleukin-6,IL-6)和肿瘤坏死因子(tumornecrosis factor,TNF-α)水平均显著升高(P<0.05),与LPS组相比,NE和DA+NE组大鼠血清IL-6和TNF-α均有不同程度的降低(P<0.05),与NE组相比,DA+NE组大鼠血清IL-6和TNF-α水平降低更显著(P<0.05)。与对照组相比,LPS、NE和DA+NE组NF-κB p65蛋白表达水平均显著升高(P<0.05),与LPS组相比,NE和DA+NE组大鼠NF-κB p65蛋白表达均有不同程度的降低(P<0.05),与NE组相比,DA+NE组大鼠NF-κB p65蛋白表达水平降低更显著(P<0.05)。结论:多巴胺联合NE对对大鼠感染性休克所导致的急性肝损伤具有良好的保护作用。

Effects of Training Dopamine Combined with NE on Rats with Acute Liver Injury Caused by Septic Shock

Objective:To investigate the effects of DA combined with NE on liver function,inflammatory factors and NF-NF-κB p65 protein in rats with acute liver injury caused by septic shock,in order to provide a certain experimental basis for clinical treatment.Methods:A total of 48 SPF-grade healthy male SD rats were chosen as research subjects and were randomly divided into four groups,i.e.the control group(n=12),LPS group(n=12),NE group(n=12),and DA+NE group(n=12).The septic shock models were established in the LPS,NE and DA+NE group.The NE group received intravenous infusion of norepinephrine,and the DA+NE group received intravenous infusion of DA on the basis of the NE group's therapy.The liver function,inflammatory factors and NF-κB p65 protein levels of rats in each group were detected.Results:Compared with the control group,the serum AST and ALT levels of the LPS,NE and DA+NE group were significantly increased(P<0.05).Compared with the LPS group,the serum AST and ALT levels of the rats in the NE and DA+NE groups were decreased to varying degrees(P<0.05).Compared with the NE group,the serum AST and ALT levels of the DA+NE group decreased more significantly(P<0.05).Compared with the control group,the serum IL-6 and TNF-αlevels in the LPS,NE and DA+NE group were significantly increased(P<0.05).Compared with the LPS group,the serum IL-6 and TNF-αin the NE and DA+NE group decreased to varying degrees(P<0.05).Compared with the NE group,the serum IL-6 and TNF-αlevels in the DA+NE group decreased more significantly(P<0.05).Compared with the control group,the expression of NF-κB p65 protein in the LPS,NE and DA+NE group was significantly increased(P<0.05).Compared with the LPS group,the expressions of NF-κB p65 protein in the NE and DA+NE group were reduced to varying degrees(P<0.05).Compared with the NE group,the expression of NF-κB p65 protein in the DA+NE group decreased more significantly(P<0.05).Conclusion:Dopamine combined with NE has a good protective effect on acute liver injury caused by septic shock in rats.