大鼠肝移植肝上下腔静脉不同缝合方式及手术体会

目的:探讨肝上下腔静脉不同缝合方式对大鼠原位肝移植的影响.方法:选取雄性SD大鼠60对,以SD大鼠为供体和受体,随机分成A、B两组,每组30对.两组均采用改良Kamada"二袖套法"进行大鼠原位肝移植,A组采用双定点连续缝合方式吻合肝上下腔静脉,B组采用单定点连续缝合方式吻合.比较两组肝上下腔静脉吻合时长、无肝期时长以...

Different Suture Technique of Suprahepatic Vena Cava and Corresponding Surgical Experience in Rat Liver Transplantation

Objective:To investigate the effects of different suture technique of suprahepatic vena cava(SHVC)reconstruction on rat orthotopic liver transplantation(ROLT).Methods:Sixty pairs of male SD rats were randomly selected as donors and recipients.According to the random number table,the rats were equally divided into group A and group B.The modified Kamada"two-cuff"technique was used to ROLT for the both two groups.In group A,double-point running suture was taken to anastomose SHVC,while single-point running suture was adopted in group B.The SHVC reconstruction time,anhepatic time,postoperative complications and survival were compared between the two groups.Results:The donors’operation time(DOT),cuffing time(CT),anhepatic phase(AP),SHVC reconstruction time(SHVCRT)in group A were respectively 28.5(27.0-31.1)min,9.5(9.0-10.6)min,20.6±1.3 min,8.5(8.0-9.0)min,and the data in group B were respectively 30.0(27.8-32.0)min,9.0(8.6-10.0)min,18.1±1.4 min,7.0(6.5-7.4)min.There were statistical differences on AH and SHVCRT between the two groups(P<0.05).Anastomotic hemorrhage(AH)of SHVC in group A was 4/30 cases,and other complications were 6/30 cases,Of which in group B were respectively 1/30 cases and 5/30 cases(P>0.05).There were no significant differences in two-day and two-week survival rate and postoperative complications between the two groups(P>0.05).Conclusions:The single-point running suture could apparently shorten the anhepatic phase and reduce the incidence of anastomotic hemorrhage,and both anastomosis technique are proved to be reliable and feasible on ROLT.