原发性醛固酮增多症大鼠自主活动和学习记忆行为的影响

摘要 目的：探讨原发性醛固酮增多症(primary aldosteronism，PA)大鼠其自主活动和对学习记忆行为的影响。方法：8周龄健康雄SD(Sprague-Dawley)大鼠(n=30)随机分为对照组与模型组各15只。两组都皮下埋置微量渗透泵，模型组泵内灌注醛固酮，对照组泵内灌注等量的生理盐水，记录大鼠自主活动和学习记忆行为的变化情况。结果：所有大鼠均存活，模型组都造模成功，切口愈合良好。模型组造模后的收缩压高于对照组(P<0.05)，也高于造模前(P<0.05)，两组造模前后心率对比差异无统计学意义(P>0.05)。模型组造模后的逃避潜伏期与穿台次数少于对照组(P<0.05)，也少于造模前(P<0.05)。模型组造模后的自主活动次数高于对照组(P<0.05)，也高于造模前(P<0.05)。造模后模型组的鼠双微基因2(Mouse Double Microgene 2，MDM2)蛋白相对表达水平高于对照组(P<0.05)。造模后模型组的血清醛固酮含量都高对照组(P<0.05)，血清钾离子、钠离子、肾素活性低于对照组(P<0.05)。结论：原发性醛固酮增多症大鼠伴随有血清钾离子、钠离子含量降低与MDM2蛋白的高表达，从而导致大鼠出现自主活动和学习记忆行为障碍。

Effects of Autonomous Activities, Learning and Memory Behaviors in Rats with Primary Aldosteronism

ABSTRACT Objective: To investigate the effects of primary aldosteronism (primary aldosteronism, PA) rats on autonomous activities and learning and memory behavior. Methods: 8-week-old healthy male SD (Sprague-Dawley) rats(n=30) were randomly divided into control group and model group with 15 rats in each groups. Micro-osmotic pumps were implanted subcutaneously in both groups. The model group were perfused with aldosterone, and the control group were perfused with the same amount of normal saline. The changes in autonomous activities and learning and memory behavior of rats were recorded. Results: All rats were alive, the model group were successfully, and the incisions healed well. The systolic blood pressure of the model group after modeling were higher than that of the control group (P<0.05), and were also higher than before modeling(P<0.05). There were no statistically significant difference in heart rate compared between the two groups before and after modeling (P>0.05). The escape latency and the number of crossings after modeling in the model group were less than those in the control group (P<0.05), and were also less than before (P<0.05). The number of autonomous activities in the model group after modeling were higher than that in the control group (P<0.05), and were also higher than before modeling (P<0.05). After modeling, the relative expression level of Mouse Double Microgene 2 (MDM2) protein in the model group were higher than that in the control group (P<0.05). After modeling, the serum aldosterone content of the model group were higher than that of the control group (P<0.05), and the serum potassium ion, sodium ion and renin activity were lower than those of the control group (P<0.05). Conclusion: Primary aldosteronism rats are accompanied by decreased serum potassium and sodium levels and high expression of MDM2 protein, which lead to disorders of autonomous activities and learning and memory behavior in rats.