不同剂量乳化异氟醚预处理对大鼠肝脏缺血再灌注损伤的保护作用

摘要 目的：探究不同剂量乳化异氟醚预处理对大鼠肝脏缺血再灌注损伤的保护作用。方法：将48只成年雄性大鼠随机分为六组：假手术组、缺血对照组、脂肪乳组、低剂量乳化异氟醚组、中剂量乳化异氟醚组和高剂量乳化异氟醚组，每组8只。检测血清中酶的含量，观察肝细胞损伤程度，直观的反应乳化异氟醚预处理对肝脏缺血再灌注损伤的影响。结果：不同组别大鼠肝脏再灌注后ALT、AST、LDH和MDA含量，SOD活性和肝细胞坏死比例均具有显著差异，随着再灌注时间的延长，各组大鼠血清ALT、AST和LDH含量均明显增加（均P＜0.05）。再灌注后1 h、2 h和4 h中剂量乳化异氟醚组大鼠血清ALT、AST和LDH含量均显著低于缺血对照组、低剂量乳化异氟醚组和高剂量乳化异氟醚组（均P＜0.05）。中剂量乳化异氟醚组大鼠肝组织匀浆中MDA含量和肝细胞坏死比例均显著低于缺血对照组、低剂量乳化异氟醚组和高剂量乳化异氟醚组，SOD活性显著高于缺血对照组、低剂量乳化异氟醚组和高剂量乳化异氟醚组（均P＜0.05）。结论：中等剂量乳化异氟醚预处理组中血清中酶含量最低，肝组织匀浆中MDA含量最低，SOD活性水平最高，肝细胞损伤程度最轻，对大鼠肝脏缺血再灌注的保护作用最好。

Protective Effects of Different Doses of Emulsified Isoflurane Pretreatment on Liver Ischemia-reperfusion Injury in Rats

ABSTRACT Objective: To investigate the protective effect of emulsified isoflurane pretreatment on liver ischemia-reperfusion injury in rats. Methods: Forty-eight adult male rats were randomly divided into six groups: sham operation group, ischemic control group, fatty milk group, low-dose emulsified isoflurane group, medium-dose emulsified isoflurane group, and high-dose emulsified isoflurane group. Group of 8 animals. The serum enzyme content was measured, the degree of liver cell damage was observed, and the effect of emulsifying isoflurane pretreatment on liver ischemia-reperfusion injury was visually reflected. Results: The contents of ALT, AST, LDH and MDA, SOD activity and the proportion of hepatocyte necrosis after liver reperfusion in rats of different groups were significantly different. As the reperfusion time prolonged, the serum ALT, AST and LDH of each group of rats were significantly different. The contents were significantly increased (all P<0.05). The serum ALT, AST, and LDH levels in the medium-dose emulsified isoflurane groups at 1 h, 2 h, and 4 h after reperfusion were significantly lower than those in the ischemic control group, low-dose emulsified isoflurane group, and high-dose emulsified isoflurane group (all P <0.05). The MDA content and the proportion of hepatocyte necrosis in the liver tissue homogenate of the medium-dose emulsified isoflurane group were significantly lower than those in the ischemic control group, the low-dose emulsified isoflurane group and the high-dose emulsified isoflurane group, and the SOD activity was significantly higher In the ischemic control group, the low-dose emulsified isoflurane group and the high-dose emulsified isoflurane group (all P<0.05). Conclusion: The medium-dose emulsified isoflurane pretreatment group had the lowest enzyme content in serum, the lowest MDA content in liver tissue homogenate, the highest SOD activity level, and the least degree of hepatocyte damage, and it had protective effects on liver ischemia-reperfusion in rats. the best.