miR-125a-5p转染对肝癌细胞增殖、侵袭、迁移的影响及相关机制研究

摘要 目的：探究miR-125a-5p转染对肝癌细胞增殖、侵袭、迁移的影响及相关机制。方法：将肝癌细胞分为对照组、下调组和上调组，并通过细胞转染建立稳定转染的下调组和上调组。MMT法检测细胞增殖能力，流式细胞仪检测细胞凋亡能力，Transwell小室实验检测细胞侵袭能力，细胞划痕实验检测细胞迁移能力，Western blot法检测P13K/Akt通路中AKT、Bax、Bcl-2、P13K、P-AKT蛋白表达量。结果：与上调组相比，下调组24、48、72 h细胞增殖率，细胞侵袭、迁移细胞数，AKT、Bcl-2、P13K、P-AKT蛋白表达量显著降低，具有统计学差异（29.67±9.87 vs 17.34±5.71，t=5.192，P＜0.05、34.75±11.56 vs 15.17±5.04，t=7.365，P＜0.05、38.48±12.81 vs 12.51 ±4.13，t=9.153，P＜0.05，72.53±24.17 vs 36.28±12.07，t=6.365，P＜0.05、86.51±28.75 vs 46.28±15.32，t=5.858，P＜0.05，1.26±0.41 vs 0.81±0.26，t=4.397，P＜0.05、1.35±0.44 vs 0.76±0.24，t=5.584，P＜0.05、1.48±0.46 vs 0.79±0.26，t=6.194，P＜0.05、1.22±0.39 vs 0.73±0.24，t=5.584，P＜0.05）；与上调组相比，下调组24、48、72h细胞凋亡率，Bax蛋白表达量显著升高，具有统计学差异（17.62±5.84 vs 29.31±9.75，t=4.879，P＜0.05、14.97±4.65 vs 34.19±11.36，t=7.427，P＜0.05、11.26±3.74 vs 38.62±12.86，t=9.690，P＜0.05，0.75±0.24 vs 1.33±0.43，t=5.587，P＜0.05）。结论：下调miR-125a-5p的表达，可通过作用于P13K/Akt通路，调控AKT、Bax、Bcl-2、P13K、P-AKT蛋白表达量，进而起到抑制肝癌细胞增殖、促进肝癌细胞凋亡以及抑制肝癌细胞的侵袭、迁移能力。

Study on the Effect of miR-125a-5p Transfection on the Proliferation, Invasion and Migration of Hepatoma Cells

ABSTRACT Objective: To explore the effect of miR-125a-5p transfection on the proliferation, invasion and migration of hepatoma cells. Methods: Liver cancer cells were divided into control group, down-regulation group and up-regulation group, and stable down-regulation group and up-regulation group were established by cell transfection. Cell proliferation was detected by MMT, apoptosis was detected by flow cytometry, cell invasion was detected by Transwell chamber test, cell migration was detected by cell scratch test, and protein expression of Akt, Bax, Bcl-2, P13K and p-Akt in P13K / Akt pathway was detected by Western blot. Results: Compared with the up-regulated group, the down-regulated group had significantly lower cell proliferation rates at 24, 48 and 72 h, cell invasion and cell migration, and the expression levels of AKT, bcl-2, P13K and p-akt were significantly lower in the down-regulated group (29.67±9.87 vs 17.34±5.71, t=5.192, P<0.05, 34.75±11.56 vs 15.17±5.04, t=7.365, P<0.05, 38.48±12.81 vs 12.51 ±4.13, t=9.153, P<0.05). 72.53±24.17 vs 36.28±12.07, t=6.365, P<0.05, 86.51±28.75 vs 46.28±15.32, t=5.858, P<0.05, 1.26±0.41 vs 0.81±0.26, t=4.397, P< 0.05, 1.35±0.44 vs 0.76±0.24, t=5.584, P<0.05, 1.48±0.46 vs 0.79±0.26, t=6.194, P<0.05, 1.22±0.39 vs 0.73±0.24, t=5.584, P<0.05); Compared with the up-regulated group, the apoptosis rate of 24, 48, and 72h cells in the down-regulated group was significantly increased, with statistically significant differences (17.62±5.84 vs 29.31±9.75, t=4.879, P<0.05, 14.97±4.65 vs 34.19±11.36, t=7.427, P<0.05, 11.26±3.74 vs 38.62±12.86, t=9.690, P<0.05, 0.75±0.24 vs 1.33±0.43, t=5.587, P<0.05). Conclusion: The down-regulation of mir-125a-5p can regulate the expression of Akt, Bax, Bcl-2, P13K and p-Akt protein by acting on P13K / Akt pathway, and then inhibit the proliferation, apoptosis, invasion and migration of hepatoma cells.