小檗碱联合松果菊苷抗人脑胶质细胞衰老的实验研究

摘要 目的：探究小檗碱联合松果菊苷抗人脑胶质细胞衰老的作用及其分子机制。方法：采用依托泊苷诱导人脑胶质细胞衰老，使用小檗碱与松果菊苷联合干预，采用β-半乳糖苷酶染色法色观察衰老阳性细胞比例，采用CCK-8法检测细胞活力，采用流式细胞术检测细胞周期，采用Western blot法检测细胞中衰老相关蛋白FoxO1、CAT、SOD2、 Bcl-2的表达水平。结果：小檗碱联合松果菊苷可显著减少依托泊苷诱导的衰老阳性细胞比例，显著增强细胞活力，显著降低G2/M期细胞比例，显著提高FoxO1、CAT、SOD2、Bcl-2的蛋白水平。结论：小檗碱联合松果菊苷可显著抑制依托泊苷诱导的人脑胶质细胞衰老，可能与增强细胞活力、推进细胞周期进程、降低胞内氧化应激水平、抗凋亡等途径有关。

Experimental Study of Berberine Combined with Echinacoside on the Senescence of Human Brain Glial Cells

ABSTRACT Objective: To investigate the anti-aging effect and molecular mechanismof Berberine combined with Echinacoside on human glial cells. Methods: Etoposide was used to induce senescence of human glial cells. Berberine and Echinacoside were combined to treat. The proportion of positive cells was tested by beta-galactosidase staining. Cell viability was detected by CCK-8 method. Cell cycle was detected by flow cytometry. The expression levels of senescence-related proteins FoxO1, CAT, SOD2 and Bcl-2 were detected by Western blot. Results: Berberine combined with Echinacoside could significantly reduce the proportion of etoposide-induced positive cells, sensibly enhance cell viability, markedly reduce the proportion of cells in G2/M phase, and significantly increase the expression levels of FoxO1, CAT, SOD2 and Bcl-2. Conclusion: Berberine combined with Echinacoside can significantly inhibit etoposide-induced senescence of human glial cells, which may be related to enhancing cell viability, promoting cell cycle progression, reducing intracellular oxidative stress level, and anti-apoptosis.