鱼类双重荧光标记研究——以中华倒刺鲃幼鱼为例

为探究盐酸四环素(TCH)和茜素红S(ARS)对中华倒刺鲃(Spinibarbus sinensis Bleeker)的影响及标记效果, 丰富鱼类荧光标记模式, 研究使用TCH(100—500 mg/L)和ARS(100—300 mg/L)对中华倒刺鲃幼鱼进行双重浸染荧光标记, 实验共设置5个处理组和1个对照组, 浸染时间均为24h。结果显示, 经90d的养殖实验后, 标记鱼的耳石(包括矢耳石和星耳石)、倒刺、鳍条和鳍棘均能检测到双重荧光标记环, 且TCH产生的黄色荧光环比ARS产生的红色荧光环更接近骨质结构内部。较高浓度处理组的矢耳石(≥300 mg/L TCH和≥150 mg/L ARS)、星耳石(≥300 mg/L TCH 和 ≥200 mg/L ARS)和倒刺(400—500 mg/L TCH 和150—300 mg/L ARS)中均能检测到明显的标记环(n≥2), 但所有处理组的侧线鳞和非侧线鳞的荧光标记不明显(0≤n≤1)。经200—500 mg/L TCH和150—300 mg/L ARS处理的鳍条, 及经300—500 mg/L TCH和200—300 mg/L ARS处理的鳍棘中可以同时检测到明显的TCH和ARS的标记环(n≥2)。此外, 在整个实验中各处理组标记鱼与对照组相比, 在生长和存活率方面均无显著性差异(P>0.05)。结果表明, 使用TCH和ARS双重标记中华倒刺鲃幼鱼是可行的。双重荧光标记方法在水生生物标记回捕实验及实验性生物学研究方面具有潜在的应用前景。

STUDY ON DOUBLE FLUORESCENCE MARKING OF SPINIBARBUS SINENSIS JUVENILE

In order to explore the effects of tetracycline hydrochloride(TCH)and alizarin red S(ARS)on Spinibarbus sinensis and their marker effects,and to enrich the fluorescent marking patterns of fish,TCH(from 100 to 500 mg/L)and ARS(from 100 to 300 mg/L)were used for double immersion marking juvenile Spinibarbus sinensis with 6 experimental groups for 24h.The results indicated that 24h double immersion produced detectable double marks in otoliths(sagittae and asteriscus),barbs,fin rays,and fin spines after 90 days in the laboratory growth experiment,with the exception of parts of scales.Yellow fluorescent rings produced by TCH were closer to the inside of the bony structures than red fluorescent rings produced by ARS.Sagittae(≥300 mg/L TCH and≥150 mg/L ARS),asteriscus(≥300 mg/L TCH and≥200 mg/L ARS),and barbs(400—500 mg/L TCH and 150—300 mg/L ARS)showed acceptable fluorescent marks at higher concentrations(n≥2).The fluorescence marking of lateral scales and non-lateral scales were not obvious in all treatment groups(0≤n≤1).Fin rays treated with 200—500 mg/L TCH and 150—300 mg/L ARS and fin spines treated with 300—500 mg/L TCH and 200—300 mg/L ARS simultaneously had both acceptable TCH and ARS marks(n≥2).In addition,double fluorescence labeling did not impact fish survival or growth(P>0.05)in the present study.The results suggest that double immersion with TCH and ARS is suitable for double mass-marking juvenile S.sinensis,and these double marks are useful in the experimental development of biological research or restocking methodologies.